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lentivector expressing 2 guide rnas (grnas) targeting ocln, a cas9-specific tracrrna and the cas9 enzyme  (Addgene inc)


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    Addgene inc lentivector expressing 2 guide rnas (grnas) targeting ocln, a cas9-specific tracrrna and the cas9 enzyme
    Lentivector Expressing 2 Guide Rnas (Grnas) Targeting Ocln, A Cas9 Specific Tracrrna And The Cas9 Enzyme, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentivector expressing 2 guide rnas (grnas) targeting ocln, a cas9-specific tracrrna and the cas9 enzyme/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    lentivector expressing 2 guide rnas (grnas) targeting ocln, a cas9-specific tracrrna and the cas9 enzyme - by Bioz Stars, 2026-04
    90/100 stars

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    Western blot analysis of BDH2 expression in four Bdh2 -deficient HEK293T clonal cell lines ( Bdh2 -KO) and WT HEK293T cells (WT). The KO clonal cell lines (A12, A15, B9, and B13) were generated by the CRISPR–Cas9 gene-inactivation procedure. The Western blot analysis was carried out using 40 μg of the cell lysate protein, a primary rabbit antibody against the human BDH2 (catalog no.: PA5-44760; Invitrogen), and a horseradish peroxidase–conjugated goat anti-rabbit secondary antibody. The secondary antibody was detected by measuring enhanced chemiluminescence. The presence of a nonspecific signal (≈30 kDa) is in agreement with the specification of the primary antibody. BDH2, type 2 ( R )-β-hydroxybutyrate dehydrogenase; HEK293T, human embryonic kidney 293T cell line.

    Journal: The Journal of Biological Chemistry

    Article Title: Recharacterization of the mammalian cytosolic type 2 ( R )-β-hydroxybutyrate dehydrogenase as 4-oxo- l -proline reductase (EC 1.1.1.104)

    doi: 10.1016/j.jbc.2022.101708

    Figure Lengend Snippet: Western blot analysis of BDH2 expression in four Bdh2 -deficient HEK293T clonal cell lines ( Bdh2 -KO) and WT HEK293T cells (WT). The KO clonal cell lines (A12, A15, B9, and B13) were generated by the CRISPR–Cas9 gene-inactivation procedure. The Western blot analysis was carried out using 40 μg of the cell lysate protein, a primary rabbit antibody against the human BDH2 (catalog no.: PA5-44760; Invitrogen), and a horseradish peroxidase–conjugated goat anti-rabbit secondary antibody. The secondary antibody was detected by measuring enhanced chemiluminescence. The presence of a nonspecific signal (≈30 kDa) is in agreement with the specification of the primary antibody. BDH2, type 2 ( R )-β-hydroxybutyrate dehydrogenase; HEK293T, human embryonic kidney 293T cell line.

    Article Snippet: This vector encodes an enhanced specificity Cas9 enzyme (a gift from Andrea Németh; Addgene; plasmid no.: 101039) ( ).

    Techniques: Western Blot, Expressing, Generated, CRISPR